Recombinant Protein Kinases
Kinases phosphorylate molecules that control complex cellular processes, such as growth, proliferation, differentiation, motility, and apoptosis. Under pathological conditions, kinase activity can be dysregulated, and disruption of the intracellular signaling networks controlled by these enzymes leads to many diseases, such as inflammation and cancer. As a result, kinase inhibitor drugs that block the action of pathologically dysregulated kinases have become an important target for drug discovery.
Recombinant protein kinases are produced using recombinant DNA technology, in which DNA sequences encoding the kinase of interest are cloned and expressed in a host cell, such as bacteria or mammalian cells. This allows for producing large amounts of pure protein kinase, which can then be used for various research applications, such as drug discovery and kinase activity assays.
Recombinant protein kinases are commonly used in drug discovery to screen small molecule compounds for their ability to inhibit or activate kinase activity, which is important for developing kinase-targeted therapies. They are also used in biochemical assays to study kinase signaling pathways and protein-protein interactions.
In cooperation with Carna Biosciences, we offer > 300 recombinant protein kinases produced in various forms, including full-length proteins or truncated forms containing only the catalytic domain. All products are developed and produced entirely in-house from gene cloning, expression, and purification and undergo rigorous quality control. Each kinase construct is carefully selected from the literature, and the DNA sequence is confirmed before expression.
Recombinant protein kinases can be engineered to include specific modifications, such as mutations or tags, which can alter their activity, and stability, or allow for easier purification. Various means are used to activate the kinases, like co-expression with an upstream kinase, ATP treatment, dephosphorylation, or tag removal. We will explain the different methods in more detail here.
Why choose Carna`s Kinases?
Features of our recombinant protein kinases are no lag phase activity, GST-tagged or biotinylated (DYKDDDDK tagged) , available from 5 µg sizes up to bulk amounts, and batch-to-batch consistency. All products are developed and produced entirely in-house and undergo rigorous quality control, and are delivered to you with a lot specific data sheet. Take advantage of the lessons we learned and the expertise we gained over 20 years.
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We offer custom production services for any kinase of interest. Just contact us for more information.