Our Products:
Kinases and Substrates
In cooperation with Carna Biosciences we provide a diverse portfolio of over 300 active recombinant human protein and lipid kinases. Key offerings include:
- Active kinases for activity assays
- Biotinylated kinases for binding studies
- Inactive mutant kinases suitable as substrates
- Additional recombinant protein substrates
All products are developed and produced entirely in-house from gene cloning, expression, and purification, and undergo rigorous quality control. Each product undergoes rigorous quality control to guarantee reliability and performance.
Kinases
Discover a wide range of recombinant human wild-type kinases, including mutant forms, isoforms, and inactive variants, tailored for your research needs. Each kinase constructs is carefully selected from reputable scientific literature, with DNA sequences thoroughly cofirmed prior to expression.
Activation Methods
- Co-expression with upstream kinases
- ATP treatment
- Dephosphorylation
- Tag removal
Rigorous Quality Control
Every kinase undergoes comprehensive quality assurance to meet the highest standards:
- Amino acid sequence confirmation via peptide mass fingerprinting
- Purity analysis using SDS-PAGE
- Protein concentration determination through the Bradford method
- Activity assessment with substrates from Carnas' extensive library
Key Features of Our Kinases
- Activity with no lag phase
- mostly GST-tagged (partly HIS and other tagged)
- Available in flexible quantities: from 5 µg samples to bulk orders
- Batch-to-batch consistency
Select by Target Group
Select by Tag
Didn`t find what you were looking for?
We offer custom production services for any kinase of interest.
Biotinylated Kinases
We offer a wide range of biotinylated kinases labeled with a single biotin at the N-terminus, providing versatile options for various research applications.
Challenges in Biotin-Streptavidin Assays
The immobilization of target proteins onto sensor surfaces, while maintaining their structure and activity, has traditionally been a challenge and rate-limiting for the acquisition of meaningful data from assays like Surface Plasmon Resonance (SPR), BioLayer Interferometry (BLI) or other biotin-streptavidin interaction assays in small molecule drug discovery. Our biotinylated kinases overcome these limitations with:
Key Advantages of Our Biotinylated Kinases
- In vivo biotinylation during expression to maintain kinase activity and structural integrity
- High sensitivity with inimized interference with cellular proteins, ensuring reliable data and low background
- Reliable and rapid data acquisition for small molecule drug discovery
- Ease of immobilization: Proteins can be efficiently mobilized onto sensor surfaces without compromising their structure or activity.
- Purity and Quality Assurance: Amino acid sequences are verified using peptide mass fingerprinting. Purity levels exceeding 80%, tested via Coomassie Brilliant Blue (CBB) staining.
Kinase Substrates
Carna’s high-quality protein substrates are specifically designed for use in kinase activity assays, ensuring reliable and reproducible results.
In-House Development and Production
Each substrate is developed and produced in-house from gene cloning, expression in E.Coli, and purification, and undergo rigorous quality control ensuring quality and consistency.
Flexible Sizing Options
Our kinase substrates are available in a range of sizes, from small samples to bulk production, to meet the needs of every research application. Each order is accompanied by a lot-specific data sheet for full transparency and traceability.
Kinase Inhibitors
I cooperation with Selleck Chemicals we offer a comprehensive selection of kinase inhibitors targeting multiple signaling pathways essential for cancer, inflammation, and other diseases.
These inhibitors are available as part of customizable libraries, making them suitable for both drug discovery and targeted research efforts.
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