Variuos recombinant Human Wild Type Kinases and mutant forms, isoforms, or inactive forms are available right now. Each kinase construct is carefully selected from literature, and the DNA sequence is confirmed prior to expression. Various means are used to activate the kinases, e.g. co-expression with an upstream kinase, ATP treatment, dephosphorylation or tag removal.
The quality control includes confirmation of amino acid sequence by peptide mass fingerprinting, checking the purity by SDS-PAGE, determination of concentration using Bradford, and determination of activity using substrates from Carnas extensive substrate library.
Further features of our kinases are: no lag phase activity, mostly GST-tagged, available from 5 µg sizes up to bulk amounts, batch-to-batch consistency.
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